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4S Green Plus Nucleic Acid Gel Stain

  • 商品编号:002
  • 销 售 价:¥298.00
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详细说明书

产品参数

Name 4S Green Plus Nucleic Acid Gel Stain
CAT#1CAS#N/A
StorageDesiccated dark freezerShelf Life24 months
Ex (nm)1Em (nm)1
MW1SolventDMSO

产品介绍

General Description

The 4S Green Plus Nucleic Acid Stain, 10,000x in water, is a non-toxic and non-carcinogenic alternative to EB (Ethidium bromide) used for the detection of nucleic acids in agarose gels, it is as sensitive as EB, while not supplied in toxic DMSO but in water.

Detection

The 4S Green Plus Nucleic Acid Stain has fluorescence excitation maxima at 295 nm and 490 nm. It is similar to EB when bound to DNA at 530 nm.

Detect DNA bands under UV illumination, while yellow or green gelatin or cellophane

is recommended for clearer bands. Besides, non-UV LED illuminators such as blue light LED illumination could also be used.

Usage

The methods of pre-stain and post-stain could both be used.
l PRE-STAIN
Add 4S Green Plus to melted agarose when it has cooled to 50 to 60°C.

Use 8~12 μl per 100 ml of agarose or 4~6μl per 50 ml of agarose.
l POST-STAIN
Use 20~30 μl per 100 ml of staining solution, which is similar to EB. For an average gel thickness of about 7 mm, stain

30 minutes, followed by a destain of 30 minutes in water. Please protect gel and staining solution from light with aluminum foil or place in dark.